Species: Human Host Species: HEK293 (human) Genbank #: NM_018945Background:Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP byhydrolyzing cAMP and cGMP to their inactive 5 monophosphates. These cyclicnucleotides play an important role as second messengers in diverse physiologicalfunctions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)have been identified. PDE7 is widely expressed in various tissues, with PDE7A foundprimarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7Bare detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads toelevated intracellular level of cAMP. Description:Recombinant HEK293 cell line expressing human PDE7B (phosphodiesterase 7B, accession number NM_018945). Human PDE7B has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7B expression was confirmed by Western blotting. The function of expressed PDE7B was validated by cAMP detection assay. The regulation of intracellular level of cAMP by PDE7B in PDE7B stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains the luciferase gene that is under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the luciferase reporter whereas hPDE7B-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase.UniProt Q9NP56 Synonym(s): PDE7B, phosphodiesterase 7BSupplied As: Each vial contains ~1 X 10
6 cells in 1 ml of 10% DMSO.Mycoplasma Testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.Format: aqueous solution containing DMSOInstructions for use: See data sheet for detailed culturing and assay protocol.Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s): Useful to monitor cAMP and PDE7B signaling pathway activity and to screen activators or inhibitors of PDE7B.Reference(s): 1. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-11732. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117Notes:
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License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Warning(s): Avoid freeze/thaw cyclesScientific Category: PDEProduct Type: Cell LineData shown is lot-specific. Contact us for specific information on other lots.
