Bpsbioscience/BRD4 (BD1+BD2) TR-FRET Assay Kit/32612/384 reactions

来自 : 发布时间：2024-05-14

Description:The BRD4 (BD1 + BD2) TR-FRET Assay Kit is designed to measurethe inhibition of BRD4 (BD1 + BD2) binding to its substrate in a homogeneous 384reaction format. This FRET-based assay requires no time-consuming washing steps,making it especially suitable for high throughput screening applications. The assayprocedure is straightforward and simple; a sample containing terbium-labeled donor,dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes.Then, the fluorescence intensity is measured using a fluorescence reader. Synonym(s): BRD4, Bromodomain, HUNK1, MCAP Supplied As: Kit comes in a convenient format with purified BRD4 (BD1 and BD2), ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions. Format:

COMPONENTS:

Instructions for use: See assay kit data sheet for detailed protocol. Storage / Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Great for screening small molecular inhibitors for drug discovery and HTS applications. Reference(s): Filippakopoulos, P., et al., Cell 2012; 149:214. Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips

Warning(s): Avoid freeze/thaw cycles. Scientific Category: Bromodomain Product Type: Assay Kit Data shown is lot-specific. Contact us for specific information on other lots.