Bpsbioscience/NEK3, GST-tag/40140/10 µg

来自 : 发布时间：2024-05-15

Species: Human Host Species: Sf9 insect cells MW: 86 kDa Genbank #: NM_002498 Tag(s): N-terminal GST-tag a.a: full length Background:NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle (1). During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects (2). Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Description:Recombinant human NEK3, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. Synonym(s): HSPK36, MGC29949 Purity: ≥75% Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1.Kimura, M. et al: Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). Cytogenet Cell Genet. 2001;95(3-4):177-82.2.Miller, S.L. et al: Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 2007 Jul 12;26(32):4668-78. Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Serine-Threonine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.