Bpsbioscience/NAD+, Biotin-Labeled/80610/500 µl

来自 : 发布时间：2025-05-10

MW: 1180.1 Da CAS Registry #: 53-84-9 (NAD+) Background:Activated PARP cleaves NAD+ to nicotinamide and ADP-ribose and polymerizes the ADP-ribose on nuclear acceptor proteins such as histones, transcription factors, and PARP itself. Biotinylated NAD+ (6-Biotin-17-NAD+) provides a non-isotopic alternative to radiolabeled NAD+ for assays requiring this substrate. Biotinylated NAD+ can be used as an indirect measure of PARP activity when biotin incorporation is detected using a streptavidin detection system. Biotinylated NAD+ is also useful for labeling or purifying proteins via poly (ADP)ribosylation. Description:NAD+ covalently labeled with biotin (6-biotin-17-nicotinamide-adenine-dinucleotide), or NAD+ Biotin-Labeled. Can be used as a substrate by poly(ADP-ribosyl) polymerase (PARP) enzymes for generating biotin-labeled ADP-ribose monomers in PARylation reactions. Synonym(s): 6-Biotin-17-NAD+, Biotin Nicotinamide adenine dinucleotide, β- Nicotinamide-N6-(2-(6-[biotinyl] aminohexanoyl) aminoethyl) adenine dinucleotide, NAD Purity: ≥95% by HPLC Supplied As: 250 µM aqueous solution Format: Aqueous buffer solution Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed

Application(s): Useful for the study of PARP enzyme activity and DNA damage, or to purify ADP-ribosylated enzymes. Reference(s):

1. Bakondi, E., et al. J Histochem Cytochem January 2002 vol. 50 no. 1 91-98. 2. Zhang, J. (1997). Meth. Enzymol. 280: 255-65. 3. Till, S., and Ladurner, A.G. (2009). Front Biosci. 14:3246-3258. 4. Ying, W. (2007). Front Biosci. 12:1863-88.5. Ying, W. (2008). Antioxidants & Redox Signaling. 10(2):179-206.

Application Reference(s): 1. Structure-function analyses of a pertussis-like toxin from pathogenic Escherichia coli reveal a distinct mechanism of inhibition of trimeric G-proteins (2017)2.Novel PARP1/2 inhibitor mefuparib hydrochloride elicits potent in vitro and in vivo anticancer activity, characteristic of high tissue distribution (2017)2.Activity-based assay for human mono-ADP-ribosyltransferases ARTD7/PARP15 and ARTD10/PARP10 aimed at screening and profiling inhibitors (2013)

Warning(s): Avoid freeze/thaw cycles Scientific Category: PARP Product Type: Substrate Data shown is lot-specific. Contact us for specific information on other lots.