Bpsbioscience/TAK1-TAB1，GST标签/40279/10µg
商品编号：
40279
品牌：
bpsbioscience
市场价：
¥7700.00
美元价：
4620.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:74kDa
Genbank#:NM_003188,NM_006116
Tag(s):N-terminalGST-tag
a.a:TAK1(1–303),TAB1(437–end)
Background:
TAK1isaserine/threonineproteinkinasethatmediatessignalingbyTGFβandmorphogeneticprotein(BMP)(1).InresponsetoIL-1,TAK1formsakinasecomplexwithTAB1andthiscomplexisrequiredfortheactivationofnuclearfactorkappaB(Nf?B).TAK1canalsoactivateMAPK8/JNKandMAP2K4/MKK4andthusplayaroleinthecellresponsetoenvironmentalstress.Tak1isessentialforthymocytedevelopmentandactivationanddeletionofTAK1preventsmaturationofsingle-positivethymocytesdisplayingCD4orCD8(2).ThymocyteslackingTAK1failtoactivateNf?BandJNKandarepronetoapoptosisuponstimulation.
Description:
RecombinantfusionproteinconsistingofhumanproteinsTAK1(TGFβ-activatedkinase1),alsoknownasMAP3K7(Mitogen-activatedproteinkinasekinasekinase7),GenbankAccession#NM_003188,a.a.1-303,andhumanTAB1(TAK1-bindingprotein),GenbankAccession#NM_006116,a.a.437-end,linkedbyasmallpeptide(DFGGGGG),andwithanN-terminalGST-tag,MW=74kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
O43318(TAK1),Q15750(TAB1)
Synonym(s):
TAK1:MAP3K7,TGF1a,TGFβ-activatedkinase1
TAB1:MAP3K7IP1,3"-Tab1,MGC57664,TAK1-bindingprotein
TAB1:MAP3K7IP1,3"-Tab1,MGC57664,TAK1-bindingprotein
AssayConditions:
Assaywasperformedinakinasebuffercontaining0.2mMDTTusingMBPsubstrate(0.1mg/ml)and100mMATP.Reactionwasdoneat30°Cfor35min.AmountofATPtransferredwascalculatedusingADP-Glo®reagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTTand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yamaguchi,K.etal.Science270:2008-2011,1995.
2.Liu,H.-H.etal.Proc.Nat.Acad.Sci.103:11677-11682,2006.
3.Sakurai,H.,etal.(2000).FEBSLett.(Netherlands)474(2-3):141–5.
4.Liu,H.-H.,etal.(2006).Proc.Natl.Acad.Sci.USA103:11677-11682.
2.Liu,H.-H.etal.Proc.Nat.Acad.Sci.103:11677-11682,2006.
3.Sakurai,H.,etal.(2000).FEBSLett.(Netherlands)474(2-3):141–5.
4.Liu,H.-H.,etal.(2006).Proc.Natl.Acad.Sci.USA103:11677-11682.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
