Bpsbioscience/鞘氨醇激酶1，His标签/40610/20µg
商品编号：
40610
品牌：
bpsbioscience
市场价：
¥7900.00
美元价：
4740.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:46.9kDa
Genbank#:NM_001142601.1
Tag(s):N-terminalHis-tag
a.a:fulllength
Description:
HumanSphingosinekinase1orSphK1(GenBankAccessionNo.AF200328),fulllengthwithN-terminalHis-tag,MW=46.9kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q9NYA1-1
Synonym(s):
SphK1,sphingosinekinase1,SPK1
SpecificActivity:
3,300pmole/min/μg
UnitDefinition:
Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1Pperminuteat37°C.
AssayConditions:
AssaywasdoneinKinasebuffercontaining20μMSphingosineand20μMATP.Reactionwasdoneat30°Cfor35min.AmountofATPtransferredwascalculatedusingAssaywasdoneinKinasebuffer(40mMTris,pH7.4,20mMMgCl2+,0.1mg/mlBSA)containing20μMSphingosineand20μMATP.Reactionwasdoneat30°Cfor35min.AmountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega)
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,10%glycerol,225mMimidazole,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Delon,C.,etal.,J.Biol.Chem279(43)44763-44774(2004).
2.Taha,TA.,etal.,FASEBJ.20(3):482-4(2005).
ApplicationReference:
RegulationofthehepatitisCvirusRNAreplicasebyendogenouslipidperoxidation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
