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商品详细Bpsbioscience/SGK3, GST-tag/40273/10 µg

Bpsbioscience/SGK3, GST-tag/40273/10 µg

商品编号： 40273

品牌： bpsbioscience

市场价： ¥7800.00

美元价： 4680.00

产地：美国(厂家直采)

公司：

产品分类：细胞因子

公司分类： cytokine

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Species:Human

HostSpecies:Sf9insectcells

MW:82kDa

Genbank#:NM_013257

Tag(s):N-terminalGST-tag

a.a:fulllength

Background:

SGK3isamemberoftheSGKfamilyandencodesaphosphoproteinwithaPX(phoxhomology)domain.PKD1canphosphorylateandactivateSGK3invitro(1).A10-foldincreaseinPKD1increasesthephosphorylationstatusofSGK3.Whenexpressedinhumanembryonickidneycells,IGF1andperoxidesignificantlyactivateSGK3,andtheactivationcouldbereducedbypreincubationwithinhibitorsofPI3kinase.Ayeast2-hybridscreenfounddirectinteractionbetweenhumanSGK3andGSK3β.SGK3phosphorylatesseveraltargetproteinsandhasaroleinneutralaminoacidtransportandactivationofpotassiumandchloridechannels(2).

Description:

Recombinantfull-lengthhumanSGK3,alsoknownasCISKandSGKL,(GenbankAccessionNo.NM_013257)withanN-terminalGST-tag,expressedinSf9insectcellsusingabaculovirusexpressionsystem.

Synonym(s):

CISK,SGKL

Purity:

≥75%

SpecificActivity:

85pmol/min/µg

AssayConditions:

5mMMOPS,2.5mMβ-glycerophosphate,5mMMgCl₂,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.IncubateSGK3with0.2mg/mlAkt(PKB)peptidesubstrate(CKRPRAASFAE)and50?M[³³P]-ATPat30°Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.

Formulation:

50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.

Format:

Aqueousbuffersolution

Storage/StABIlity:

>6monthsat-80°C.

Application(s):

Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.

Reference(s):

1.Kobayashi,T.etal:Characterizationofthestructureandregulationoftwonovelisoformsofserum-andglucocorticoid-inducedproteinkinase.Biochem.J.344:189-197,1999.
2.Gamper,N.etal:K+channelactivationbyallthreeisoformsofserum-andglucocorticoid-dependentproteinkinaseSGK.Europ.J.Physiol.445:60-66,2002.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Kinase/Serine-Threonine

ProductType:

RecombinantProtein

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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