Bpsbioscience/ROR1, GST-tag/40396/10 µg
商品编号：
40396
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:82kDa
Genbank#:NM_005012
Tag(s):N-terminalGST-tag
a.a:429–end
Background:
ROR1isaglycosylatedtypeImembraneproteinthatbelongstotheRORsubfamilyofcellsurfacereceptorsthatplayaroleinthecontrolofmostbasiccellularprocessesincludingcellproliferation,differentiation,migrationandmetabolism(1).ManyhumanbreastcancersexpresstheROR1proteinandhigh-levelexpressionofROR1inbreastadenocarcinomaisassociatedwithaggressivedisease.SilencingexpressionofROR1inhumanbreastcancercelllinesfoundtoexpressthisproteinimpairedtheirgrowthinvitroandalsoinimmune-deficientmice(2).
Description:
RecombinanthumanROR1(GenBankAccessionNo.NM_005012),a.a.429-end,withN-terminalGST-tag,MW82kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
dJ537F10.1;NTRKR1,neurotrophictyrosinekinase,receptor-related1
Purity:
≥95%
SpecificActivity:
0.2pmol/min/µg
AssayConditions:
5mMMOPS,2.5mMbeta-glycerophosphate,5mMMgCl2,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.IncubateROR1with0.25mg/mlMyelinBasicProteinsubstrateand50?M[33P]-ATPat30°Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,and25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Afzal,A.R.et.al.Hum.Mutat.22:1-11,2003.
2.Zhang,S.etal.PLoSOne.2012;7(3):e31127.
3.Channavajhala,P.,etal.,J.Biol.Chem.2003,278:47089-47097.
4.Rodriguez,C.,etal.,CellSignal2006,18(9):1376-1385.
2.Zhang,S.etal.PLoSOne.2012;7(3):e31127.
3.Channavajhala,P.,etal.,J.Biol.Chem.2003,278:47089-47097.
4.Rodriguez,C.,etal.,CellSignal2006,18(9):1376-1385.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/ReceptorTyrosine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
