Bpsbioscience/RIPK2，GST标签/40173/10µg
商品编号：
40173
品牌：
bpsbioscience
市场价：
¥7600.00
美元价：
4560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:59kDa
Genbank#:NM_003821
Tag(s):N-terminalGST-tag
a.a:1–299
Background:
RIPK2(RIP2;RICK)isadeathdomain-containingproteinkinaseencodingapredicted540-aminoacidproteinwhichcontainsanN-terminalserine/threoninekinasecatalyticdomainandaC-terminalcaspaseactivationandrecruitmentdomain.RIPK2isthoughttoregulateapoptosisinducedbytheFASreceptorpathway(1).RIPK2hasbeenshowntospecificallyinteractwiththeCARDofICE/caspase-1andthisinteractioncorrelateswiththeprocessingofpro-caspase-1andtheformationoftheactivecaspase-1p20(2).
Description:
RecombinanthumanRIPK2(GenBankAccessionNo.NM_003821),(a.a.1-299)withN-terminalGst-tag,MW=59kDa,expressedinanbaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
RICK;RIP2;CARD3;CARDIAK;CCK;GIG30
Purity:
≥75%
SpecificActivity:
20pmol/min/μg
AssayConditions:
AssayBuffer:25mMMOPS,pH7.2,12.5mMß-glycerophophate,25mMMgCl2,5mMEGTA,2mMEDTA.IncubateRIPK2withsubstrate(0.2mg/ml)and[32P]-ATPat30°Cfor15minutes,thenspotreactiononP81paper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Inohara,N.etal:RICK,anovelproteinkinasecontainingacaspaserecruitmentdomain,interactswithCLARPandregulatesCD95-mediatedapoptosis.J.Biol.Chem.273:12296-12300,1998.Note:Erratum:J.Biol.Chem273:18675only,1998.
2.Thome,M.etal:IdentificationofCARDIAK,aRIP-likekinasethatassociateswithcaspase-1.Curr.Biol.8:885-888,1998.
2.Thome,M.etal:IdentificationofCARDIAK,aRIP-likekinasethatassociateswithcaspase-1.Curr.Biol.8:885-888,1998.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
