Bpsbioscience/RIPK1, GST-Th-Tag/40371/10 µg
商品编号:
40371
品牌:
bpsbioscience
市场价:
¥7600.00
美元价:
4560.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:64kDa
Genbank#:NM_003804
Tag(s):N-terminalGST-Th-Tag
a.a:1-327
Background:
RIPK1orReceptorInteractingProteinKinase1isaserine/threoninekinasethatwasoriginallyidentifiedasinteractingwiththecytoplasmicdomainofFAS.RIPK1hasbeendeemedasanimportantelementinthesignaltransductionmachinerythatmediatesprogrammedcelldeath.RIPK1hasbeenshowntointeractwithTRADD,TRAF1TRAF2andTRAF3andTRADDcanactasanadaptorproteintorecruitRIPK1totheTNFR1complexinaTNF-dependentprocess.TNFαiscapableofactivatingthenoncanonicalNF-κBpathway,butthisactivationofthispathwayisnegativelyregulatedbyRIPK1.
Description:
HumanRIPK1,alsoknownasRCKandMAP3K19,GenBankAccessionNo.NM_003804,a.a.1-327,withN-terminalGST-tagandthrombincleavagesite,MW=108kDa,expressedinaBaculovirus-infectedSf9cellexpressionsystem.
Synonym(s):
RCK,MAP3K19,receptor-interactingserine/threonine-proteinkinase1isoform1;receptor-interactingserine/threonine-proteinkinase1;serine/threonine-proteinkinaseRIP;celldeathproteinRIP;receptor-interactingprotein1;receptor(TNFRSF)-interact
AssayConditions:
AssaywasdoneinaKinasebuffer(BPSCat.#79334)usingMBP(0.2mg/ml)asasubstratewith20μMATPat30°Cfor45minutes.TheamountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,20%glycerol,1.6mMglutathione
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hsu,H.etal.,Immunity4:387-396,1996.
2.Kim,JY.etal.,JCellSci.2011Feb15;124(Pt4):647-56.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。