Bpsbioscience/PRKG1, GST-tag/40175/10 µg
商品编号：
40175
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 100 kDa
Genbank #: NM_006258
Tag(s): N-terminal GST-tag
a.a: full length
Background:
PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain (1). By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation (2). Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP.
Description:
Recombinant human PRKG1, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s):
PGK, CGKI, PRKG1B, PRKGR1B, FLJ36117, MGC71944, cGKI-BETA, cGKI-alpha, DKFZp686K042
Purity:
≥75%
Formulation:
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Orstavik, S. et al: Characterization of the human gene encoding the type I-alpha and type I-beta cGMP-dependent protein kinase (PRKG1). Genomics 42: 311-318, 1997.
2. Li, Z. et al: A stimulatory role for cGMP-dependent protein kinase in platelet activation. Cell 112: 77-86, 2003.
2. Li, Z. et al: A stimulatory role for cGMP-dependent protein kinase in platelet activation. Cell 112: 77-86, 2003.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
