Bpsbioscience/PLK4, GST-tag/40236/10 µg
商品编号：
40236
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9InsectCells
MW:140kDa
Genbank#:NM_014264
Tag(s):N-terminalGST-tag
a.a:1-836
Description:
Humanpolo-likekinase4(PLK4),alsoknownasserine/threonineproteinkinase18(STK18),SAK,GenBankAccessionNo.NM_014264,a.a.1-836withanN-terminalGST-tag,expressedinabaculovirus-infectedSf9cellexpressionsystem.MW=140kDa.
UniProt
O00444
Synonym(s):
polo-likekinase4,serine/threonineproteinkinase18(STK18),SAK
SpecificActivity:
6.4pmol/min/μg
AssayConditions:
10μlofvaryingamountsofPLK4wasmixedwith5μlof1mg/mlMBPproteinsubstrateand5μlofdistilledwater.Reactionwasinitiatedbyadding5μlof250μM[33P]-ATPandincubatingfor15minat30°Cfollowedbyspottingthereactiononphosphocellulosepaper,fixingin1%phosphoricacid,andassayingwithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutatione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforstudyingenzymekineticsandscreeningsmallmoleculeinhibitors.
Reference(s):
1.Kleylein-Sohn,J.etal.,Dev.Cell.2007;13:190-202.
2.Ko,M.A.etal.,NatureGenet.2005;37:883-888.
2.Ko,M.A.etal.,NatureGenet.2005;37:883-888.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedproteinisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
