Bpsbioscience/PLK3, His-tag/40035/10 µg
商品编号：
40035
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:36.7kDa
Genbank#:NM_004073
Tag(s):N-terminalHis-tag
a.a:58–339
Background:
PLK3orPolo-likekinase3isacytokine-inducIBLekinasethatisamemberofthePolofamilyofserine/threonineproteinkinases.PLK3containsbothacatalyticdomainandaputativeregulatorydomainandplaysanimportantroleintheregulationofcellcycleprogressionandtumOrigenesis.PLK3iscloselyassociatedwithcentrosomesinseveralhumancelllinesanditslocalizationisdependentontheintegrityofmicrotubules.Hence,PLK3isalsoinvolvedinregulatingmicrotubuledynamicsandcentrosomalfunction.
Description:
HumanPlk3(GenBankAccessionNo.NM_004073),(a.a.58-339)withN-terminalHis-tag,MW=36.7kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.Source:BaculovirusinfectedSf9cellsPurity:≥80%bySDS-PAGE.SpecificActivity:500U/mg.Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmolofcaseinsubstrateperminuteatpH7.4and30°C.Assaybuffer:50mMHEPES,pH7.4,3mMMgCl2,3mMMnCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP.
Synonym(s):
CNK,FNK,PRK,polo-likekinase3
SpecificActivity:
350pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmolofsubstrateperminuteatpH7.5and30°C.
AssayConditions:
Enzymereactionisconductedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200µMATP,0.01%Brij-35and2µMsubstrate(SER/THR16,Invitrogen)atroomtemperaturefor1hour.
Formulation:
50mMTris-HCl,pH8.0,138mMNaCl,2.7mMKCl,0.05%Tween-20,50%glyceroland1mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Li,Z.,etal.,J.Biol.Chem.280(17),16843-16850(2005).
2.Xie,S.,etal.,Oncogene23(21),3822-3829(2004).
2.Xie,S.,etal.,Oncogene23(21),3822-3829(2004).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
