Bpsbioscience/PLK2, GST-tag/40034/10 µg
商品编号：
40034
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:107kDa(calculated)
Genbank#:NM_006622
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
PLK2orPolo-likekinase2isaserum-inducIBLekinasethatisamemberofthePolofamilyofserine/threonineproteinkinaseswhichplayanessentialroleinnormalcelldivision.PLK2phosphorylatestheP4.1-associatedprotein(CPAP),ahumanhomologueofSAS-4,inprocentrioleformationduringthecentrosomecycle.PLK2mayalsoplayaroleasatumorsuppressorsinceitinteractswithTSC1proteinwhichinturninhibitsmTORsignaling,tumorgrowthandchemosensitivityunderhypoxicconditions.HumanlungtumorcellsdeficientinPLK2growlargerthancontroltumors,andPLK2interactswithendogenousTSC1proteininthesetumors.
Description:
HumanPlk2(GenBankAccessionNo.NM_006622),fulllength,withN-terminalGST-tag,expressedinabaculovirusinfectedSf9cellexpressionsystem.TheoreticalMWis107kDa.
Synonym(s):
SNK,polo-likekinase2
SpecificActivity:
3.3pmole/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmolofsubstrateperminuteatpH7.5and30°C.
AssayConditions:
Enzymereactionisconductedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200µMATP,0.01%Brij-35and2µMsubstrate(SER/THR16,Invitrogen)atroomtemperaturefor1hour.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,18mMglutathione,10%glyceroland3mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Syed,N.etal.,Blood.2006Jan1,107(1):250-6.
2.Eckerdt,F.etal.,Oncogene.2005Jan10,24(2):267-76.
2.Eckerdt,F.etal.,Oncogene.2005Jan10,24(2):267-76.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
