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Bpsbioscience/SIRP-α / HEK293 Recombinant Cell Line/60689/2 vials
SIRPalpha(CD172A,PTPNS1,SIRPa)isamemberofthesignal-regulatory-protein(SIRP)family,whicharetransmembraneimmunoglobulinreceptorsthatnegativelyregulatereceptortyrosinekinase-coupledsignalingprocesses.SIRPalphaisexpressedpredominantlyonmacrophagesanddendriticcells.InteractionwithitsligandCD47mediatessignalsthatblocksphagocytosis(alsoknownasthe“don’teatme”signal).
Thiscelllinehasbeenscreenedusingthe(Cat.#LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Cat#LT07-518)wasusedasapositivecontrol.
ThawMedium1(BPSBioscience,Cat.#60187):MEMmedium(Hyclone,Cat.#SH30024.01)supplementedwith10%FBS(Invitrogen,#26140-079),1%non-essentialaminoacids(Hyclone,Cat.#SH30238.01),1mMNapyruvate(Hyclone,Cat.#SH30239.01),1%Penicillin/Streptomycin(Hyclone,#SV30010.01)
CompleteGrowthMedium:ThawMedium1(BPSBioscience,Cat.#60187)plus2µg/mlPuromycinDihydrochloride(ThermoFisher,Cat.#A1113803).
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LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
