RecombinantCHO-K1cellsconstitutivelyexpressinghumanPD-L1(ProgrammedCellDeath1Ligand1,CD274,B7homolog1(B7-H1),GenBankaccession#NM_014143)anengineeredTcellreceptor(TCR)activator.
PD-1/NFATreporter-Jurkatcellline(BPSCat.#60535)
Assaymedium:RPMI1640+10%FBS+1%Penicillin/Streptomycin(ThawMedium2,BPSCat.#60184)
Anti-PD-1neutralizingantibody(BPSCat.#71120)
Anti-PD-L1neutralizingantibody(BPSCat.#71213)
96-welltissueculture-treatedwhiteclear-bottomassayplate
One-Stepluciferaseassaysystem(BPSCat.#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer
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LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
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