Bpsbioscience/PKCα (PKCalpha), GST-tag/40157/10 µg
商品编号：
40157
品牌：
bpsbioscience
市场价：
¥6600.00
美元价：
3960.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:102kDa
Genbank#:NM_002737
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
PKCα(PKCalpha)isamemberoftheproteinkinaseC(PKC)familyofserine-andthreonine-specificproteinkinasesthatcanbeactivatedbycalciumandthesecondmessengerdiacylglycerol.PKC-alphahasbeenreportedtoplayrolesinmanydifferentcellularprocesses,suchascelladhesion,celltransformation,cellcyclecheckpoint,andcellvolumecontrol(1).PKCαhasbeenassignedtothechromosomeregion17q22-q23.2andhasbeenidentifiedasafundamentalregulatorofcardiaccontractilityandCa(2+)handlinginmyocytes(2).
Description:
RecombinanthumanPKCα(PKCalpha),fulllength,GenBankAccessionNo.NM_002737,withN-terminalGST-tag,expressedinSf9insectcellsviaabaculovirusexpressionsystem.MW=102kDa.
Synonym(s):
AAG6,PKCA,PRKCA,PRKACA,MGC129900,MGC129901,PKCαproteinkinasecalpha
Purity:
≥80%
SpecificActivity:
320pmol/min/µg
AssayConditions:
AssaywasdoneinKinasebuffercontaining0.1mMCaCl2andlipidactivator(1mg/mlPhosphatidylSerineand0.02mg/mlDiacylglycerolin0.3%TritonX-100)usingHistoneH1(0.1mg/ml)and20μMATP.Reactionwasdoneat30°Cfor40min.AmountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega)
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,3mMDTT,20%glycerol,1.6mMGlutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Proteinmaybedilutedto≥100μg/mlinPBS+glycerolandstoredat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Coussens,L.etal.Science233:859-866,1986.
2.Braz,J.C.etal.NatureMed.10:248-254,2004.
2.Braz,J.C.etal.NatureMed.10:248-254,2004.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
