Bpsbioscience/PIP5K1A, GST-tag/40305/10 µg
商品编号：
40305
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Baculovirus-infectedSf9insect
MW:86kDa
Genbank#:BC_007833
Tag(s):N-terminalGST-tag
a.a:Fulllength
Description:
Humanphosphatidylinositol4-phosphate5-kinasetype-1alpha(PIP5K1A)alsoknownasPIP5K1,GenBankAccessionNo.BC_007833,MW=86kDa,full-lengthexpressedinabaculovirus-infectedSf9insectcellexpressionsystem.
UniProt
Q99755
Synonym(s):
PIP5K1α
SpecificActivity:
1310pmol/min/μg
AssayConditions:
10μlofvaryingamountsofPIP5K1Awasmixedwith5μlof125μMphosphatidylinositol3,4-bisphosphatesubstrateand5μlofkinasedilutionbuffer.Reactionwasinitiatedbyadding5μlof250μMATPassaysolution,sonicatingfor10sec.andincubatingfor15minat30°C.Add25μlofADP-Glo(Promega),andincubateatroomtemp.for40min.withshakingThenadd50μlKinaseDetectionReagent(Promega),incubate30min.atroomtemp.andreadplate.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C
Application(s):
Usefulforstudyingenzymekineticsandscreeningsmallmoleculeinhibitors.
Reference(s):
1.Pan,W.,etal.,Science.2008;321:1350-1353.
2.Barrero-Villar,M.,etal.,J.Immunol.2008;15;181(10):6882-6888.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedproteinisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
