Bpsbioscience/PIM3, Active, GST-tag/41108/10 µg
商品编号：
41108
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 Insect Cells
MW: 63 kDa
Genbank #: NM_145478
Tag(s): N-terminal GST tag
a.a: full-length
Description:
Recombinant human
serine/threonine-protein kinase PIM3
(PIM3), GenBank Accession No.
NM_145478, full-length with an N-terminal
GST tag and expressed in a baculovirusinfected
Sf9 insect cell expression system.
MW = 63 kDa.
UniProt
Q86V86
Synonym(s):
serine/threonine-protein kinase PIM3
Specific Activity:
785 pmol/min/g
Assay Conditions:
Varying amounts of
enzyme were incubated in 5 mM MOPS,
pH 7.2, 2.5 mM -glycerophosphate, 4
mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA,
0.4 mM EDTA, 0.01 mM DTT, 0.05 mM
ATP, 5 μg of RSK substrate and 32 μCi of
[33P]-ATP was incubated at 30°C for 15
minutes. Then spot reaction on
phosphocellulose paper, fix in 1%
phosphoric acid, and assay with a
scintillation counter.
Formulation:
50 mM Tris-HCl, pH 7.5, 150
mM NaCl, 10 mM glutathione, 0.1 mM EDTA,
0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fujii, C. et al., Int. J. Cancer. 2005; 114:: 209-218.
2. Li, Y.-Y., et al., Cancer Res. 2006; 66: 6741-6747.
2. Li, Y.-Y., et al., Cancer Res. 2006; 66: 6741-6747.
Warning(s):
Avoid
freeze/thaw cycles. Storing diluted enzyme is
not recommended, if necessary, use carrier
protein (BSA 0.1 – 0.5%).
Scientific Category:
Kinase - Serine/Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
