Bpsbioscience/PIM1, Active, GST-tag/41107/10 µg
商品编号：
41107
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 Insect Cells
MW: 62 kDa
Genbank #: NM_002648
Tag(s): N-terminal GST tag
a.a: full-length
Description:
Recombinant human
serine/threonine-protein kinase PIM1
(PIM1), GenBank Accession No.
NM_002648, full-length with an N-terminal
GST tag and expressed in a baculovirusinfected
Sf9 insect cell expression system.
MW = 62 kDa.
UniProt
P11309
Synonym(s):
serine/threonine-protein kinase PIM1
Specific Activity:
316 pmol/min/µg
Assay Conditions:
Varying amounts of
enzyme were incubated in 5 mM MOPS,
pH 7.2, 2.5 mM -glycerophosphate, 4
mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA,
0.4 mM EDTA, 0.01 mM DTT, 0.05 mM
ATP, 10 μg of S6K synthetic peptide
substrate, and 32 μCi of [33P]-ATP was
incubated at 30°C for 15 minutes. Then
spot reaction on phosphocellulose paper,
fix in 1% phosphoric acid, and assay with
a scintillation counter.
Formulation:
50 mM Tris-HCl, pH 7.5, 150
mM NaCl, 10 mM glutathione, 0.1 mM EDTA,
0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Format:
Aqueous buffer solution.
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Meeker, T.C., et al., J. Cell. Biochem. 1987; 35(2): 105-112.
2. Friedmann, M. et al., Arch. Biochem Biophys. 1992; 298(2): 594-601.
2. Friedmann, M. et al., Arch. Biochem Biophys. 1992; 298(2): 594-601.
Warning(s):
Avoid
freeze/thaw cycles. Storing diluted enzyme is
not recommended, if necessary, use carrier
protein (BSA 0.1 – 0.5%).
Scientific Category:
Kinase - Serine/Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
