4000-520-616
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4000-520-616
当前位置: 首页 > 产品中心 > kinase > Bpsbioscience/PI3激酶(p110δ/p85α),GST标签/40628/20µg
商品详细Bpsbioscience/PI3激酶(p110δ/p85α),GST标签/40628/20µg
Bpsbioscience/PI3激酶(p110δ/p85α),GST标签/40628/20µg
Bpsbioscience/PI3激酶(p110δ/p85α),GST标签/40628/20µg
商品编号: 40628
品牌: bpsbioscience
市场价: ¥8300.00
美元价: 4980.00
产地: 美国(厂家直采)
公司:
产品分类: 激酶
公司分类: kinase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:230kDa(complex)
Genbank#:NM_005026.2(p110δ),XM_043865(p85α)
Tag(s):N-terminalGST-tag(p110δ),notagonp85α
a.a:fulllength
Description:
ComplexofN-terminalGST-taggedrecombinantfull-lengthhumanp110δ(GenBankAccessionNo.NM_005026),andrecombinantfulllength,humanp85α,GenBankAccessionNo.XM_043865).CoexpressedinaBaculovirusinfectedSf9cellexpressionsystem.p110δMW=146kDa,p85αMW=84kDa.
Synonym(s):
PIK3CD,p110delta,Phosphoinositide-3-Kinase,Catalytic,Delta,PIK3R1,Phosphoinositide-3-Kinase,RegulatorySubunit1(Alpha),GRB1,p85alpha
SpecificActivity:
520pmol/min/μg.
UnitDefinition:
Aunitofp110δ/p85αkinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofPhosphoinositol-3,4,5-Trisphosphate/minat30°C.
AssayConditions:
A25μlkinasereactionisconductedinabuffercontaining40mMTris-HCl(pH7.4),20mMMgCl2,100uMATP,200μMPI:PSsubstrate(BPScatalog#40560)andp110δ/p85αenzymefor15minutesat30°C.ATPreductionisdetectedusingADP-Glo™KinaseAssayPlatform(PromegaCorporation,MADIson,WI).
Formulation:
25mMTris,pH8.0,69mMNaCl,1.35mMKCl,0.03%Tween-20,3mMDTT,50%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Vanhaesebroecketal.(1997)Proc.Natl.Acad.Sci.U.S.A.94:4330.
2.Vanhaesebroecketal.(1999)EMBOJ.18:1292.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。