Bpsbioscience/PHKG1, GST-tag/40150/10 µg
商品编号：
40150
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 70 kDa
Genbank #: NM_006213
Tag(s): N-terminal GST-tag
a.a: full length
Background:
PHKG1 or phosphorylase kinase gamma 1 is a member of the Ser/Thr protein kinase family that encodes a protein with one protein kinase domain and two calmodulin-binding domains. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme (1). PHKG1 is the catalytic member of a 16-subunit protein kinase complex that contains equimolar ratios of 4 subunit types known as alpha, beta, gamma and delta (2). Skeletal muscle contains the highest amount of phosphorylase kinase enzymatic activity, although activity is also observed in liver, cardiac muscle, brain, and several other tissues.
Description:
Recombinant human PHKG1 (phosphorylase kinase gamma 1), GenBank Accession No. NM_006213, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s):
PHKG
Purity:
≥75%
Specific Activity:
29 pmol/min/µg
Assay Conditions:
5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate PHKG1 with 0.25 mg/ml ZIKtide peptide substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation:
50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Burwinkel, B. et.al: Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases. Europ. J. Hum. Gen. 11: 516-526, 2003.
2. Wehner, M. et.al: Human cDNA encoding the muscle isoform of the phosphorylase kinase gamma subunit (PHKG1).
2. Wehner, M. et.al: Human cDNA encoding the muscle isoform of the phosphorylase kinase gamma subunit (PHKG1).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
