Bpsbioscience/PDK1, GST-tag/40080/10 µg
商品编号：
40080
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:89kDa
Genbank#:NM_002613
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
PDK1(3-phosphoinositide-dependentproteinkinase)isactivatedbythepresenceofPtdIns(3,4,5)P3orPtdIns(3,4)P2.PDK1thenactivatesproteinkinaseB(PKB)which,inturn,inactivatesglycogensynthasekinase-3(GSK3).ThephosphorylationofotherproteinsbyPKBandGSK3islikelytomediatemanyoftheintracellularactionsofinsulin.Thus,PDK1playsakeyroleinmediatingmanyoftheactionsofthesecondmessenger(s)PtdIns(3,4,5)P3and/orPtdIns(3,4)P2.ThehumanPDK1isa556-residuemonomericenzymecomprisingofacatalyticdomainthatismostsimilartothePKA,PKBandPKCsubfamilyofproteinkinases.
Description:
Human3-phosphoinositide-dependentproteinkinase(PDK1),GenBankAccessionNo.NM_002613,fulllengthwithN-terminalGST-tag,MW=89kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
PRO0461;PDPK1;MGC20087;MGC35290;PkB-like;PkB-like1,
SpecificActivity:
≥100 pmol/min/µg
AssayConditions:
AssaywasdoneinKinasebuffercontaining0.2mMDTTusingPDKtidesubstrate(0.1mg/ml)and20μMATP.Reactionwasdoneat30˚Cfor45min.AmountofATPtransferredwasmeasuredusingKinase-Glo®reagent(Promega)
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,3mMDTTand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yang,KJ.,etal.,JBiolChem.2008Jan18,283(3):1480-91.
2.Kikani,CK.,etal.,JCellBiochem.2005Dec15,96(6):1157-62.
ApplicationReference:
PDK1controlsupstreamPI3KexpressionandPIP3generation(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
