Bpsbioscience/PAK1/40072/10µg
商品编号：
40072
品牌：
bpsbioscience
市场价：
¥7900.00
美元价：
4740.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:33.6kDa
Genbank#:NM_002576.4
Tag(s):none
a.a:248–545
Background:
PAK1isamemberofthep21-activatedkinases(PAKs)whichhavebeenimplicatedintheregulationofcellmorphology,motilityandtransformation.Theseserine/threoninekinasesareactivatedbyandareeffectorsofsmallGTPases,CDC42andRAC.PAK1belongstotheGroupIPAKswhichalsoincludesPAK2andPAK3.PAK1isakeyregulatoroftheactinCytoskeleton,adhesionandcellmotility.InactivedimericPAK1ismainlycytosolicandinteractionwiththeactivatorsCdc42-GTPandRac1-GTPstimulatesthekinaseatthesitesofcellularprotrusionsformingadhesionstotheextracellularmatrix.
Description:
HumanPAK1(GenBankAccessionNo.NM_002576.4),a.a.248-545,MW=33.6kDa,expressedinE.coli.
Purity:
≥95%
SpecificActivity:
32638pmoles/min/μg
AssayConditions:
AnalysisofenzymaticactivitywasperformedaccordingtoZlyteassayprotocol(Invitrogen).PAK1wasincubatedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200μMATP,0.01%Brij-35,and2μMsubstrate(SER/THR14,Invitrogen)atroomtemperaturefor1hour.Developersolutionwasaddedtoreactionfor1houratRT.Fluorescencewasdetectedusingλexc=460±40nmandλem=528±20nm
Formulation:
25mMTris-HCl,pH8,150mMNaCl,10%glycerol,5mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Sheehan,K.A.,etal.,Am.J.Physiol.,CellPhysiol.296(1),C47-C58(2009).
2.Reddy,S.D.,etal.,CancerRes.68(20),8195-8200(2008).
2.Reddy,S.D.,etal.,CancerRes.68(20),8195-8200(2008).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
