Bpsbioscience/大鼠γ干扰素/90164-A/20µg
商品编号:
90164-A
品牌:
bpsbioscience
市场价:
¥2600.00
美元价:
1560.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Rat
HostSpecies:E.coli
MW:16kDa
Genbank#:P01581
a.a:23–156
Background:
IFN-gammaisproducedmainlybyT-cellsandnaturalkillercellsactivatedbyantigens,mitogens,oralloantigens.ItisproducedbylymphocytesexpressingthesurfaceantigensCD4andCD8.IFNgammareceptorsareexpressedonalltypesofhumancellswiththeexceptionofmatureerythrocytes.IFN-gamma-receptorcomplexesarerapidlyinternalizedbyendocytosis.IFN-gammahasantiviralandantiparasiticactivitiesandalsoinhibitstheproliferationofanumberofnormalandtransformedcells.Interferon-gammasynergiseswithTNF-alphaandTNF-betaininhibitingtheproliferationofvariouscelltypes.ThegrowthinhibitoryactivitiesofIFN-gammaaremorepronouncedthanthoseoftheotherinterferons.However,themainBIOLOGicalactivityofIFN-gammaappearstobeimmunomodulatoryincontrasttotheotherinterferonsthataremainlyantiviral.InT-helpercells,IL-2inducesthesynthesisofIFN-gammaandothercytokines.IFN-gammaactssynergisticallywithIL-1andIL-2andappearstoberequiredfortheexpressionofIL-2receptorsonthecellsurfaceofT-lymphocytes.BlockingoftheIL-2receptorbyspecificantibodiesalsoinhibitsthesynthesisofIFN-gamma.
Description:
RecombinantInterferongammaisadisulfide-linkedmonomerproteinconsistingof135aminoacidresidues,andmigratesasanapproximately16kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingratInterferon-gammamaturechainwasexpressedinE.coli.
UniProt
P01581
Synonym(s):
IFNG,Interferongamma,IFG,IFN
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50wasdeterminedbythecytopathicinhibitionassaywithmurineL929cellsinfectedbyEMCvirus,andwasfoundtobe<1ng/ml,correspondingtoaspecificactivityof>1x106units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredPBS,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.SikorskiK,etal.CytokineGrowthFactorRev.2011Aug,22(4):211-9.
2.GysemansC,etal.BiochemSocTrans.2008Jun,36(Pt3):328-33.
3.RameshwarP.CellRes.2008Aug,18(8):805-6.
2.GysemansC,etal.BiochemSocTrans.2008Jun,36(Pt3):328-33.
3.RameshwarP.CellRes.2008Aug,18(8):805-6.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
QGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAKkDaFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。