Bpsbioscience/大鼠γ干扰素/90164-B/100µg
商品编号：
90164-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Rat
Host Species: E. coli
MW: 16 kDa
Genbank #: P01581
a.a: 23–156
Background:
IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes. IFN-gamma-receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. In T-helper cells, IL-2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL-1 and IL-2 and appears to be required for the expression of IL-2 receptors on the cell surface of T-lymphocytes. Blocking of the IL-2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.
Description:
Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 135 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interferon-gamma mature chain was expressed in E. coli.
UniProt
P01581
Synonym(s):
IFNG, Interferon gamma, IFG, IFN
Purity:
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the cytopathic inhibition assay with murine L929 cells infected by EMC virus, and was found to be < 1 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
Formulation:
Lyophilized from 0.2 µm filtered PBS, pH 7.0.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. Sikorski K, et al. Cytokine Growth Factor Rev. 2011 Aug,22(4):211-9.
2. Gysemans C, et al. Biochem Soc Trans. 2008 Jun,36(Pt 3):328-33.
3. Rameshwar P. Cell Res. 2008 Aug,18(8):805-6.
2. Gysemans C, et al. Biochem Soc Trans. 2008 Jun,36(Pt 3):328-33.
3. Rameshwar P. Cell Res. 2008 Aug,18(8):805-6.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
QGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAK kDaFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
