Bpsbioscience/p70S6Kb, GST-tag/40146/10 µg
商品编号：
40146
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:85kDa
Genbank#:BC000094
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
p70S6KbisamemberoftheRSK(ribosomalS6kinase)familyofserine/threoninekinasesandisactivatedbymitogenicstimuli,includinggrowthfactors,cytokines,andphorbolesters.p70S6Kbcontains2nonidenticalkinasecatalyticdomainsandphosphorylatestheS6ribosomalproteinandeucaryotictranslationinitiationfactor4B.PhosphorylationofS6leadstoanincreaseinproteinsynthesisandcellproliferation.PI3kinasepathwayandmTORareinvolvedintheactivationofp70S6Kbbutotherpathwayscanalsoactivatethistargetprotein.
Description:
Recombinanthumanp70S6Kb,alsoknownasRPS6KB2,STK14B,KLS,P70-beta,orSRK,GenBankAccessionNo.BC_000094,fulllengthwithanN-terminalGSTtagandexpressedinabaculovirus-infectedSf9insectcellexpressionsystem.MW=85kDa.
Synonym(s):
RPS6KB2,STK14B,KLS,P70-beta,P70-beta-1,P70-beta-2,S6K2,p70(S6K)-beta,S6K-beta2,SRK
Purity:
≥90%
SpecificActivity:
107pmol/min/µg
AssayConditions:
5mMMOPS,2.5mMβ-glycerophosphate,5mMMgCl2,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.Incubatep70S6Kwith0.2mg/mlRSK-subpeptidesubstrateand50?M[33P]-ATPat30°Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Gout,I.etal:Molecularcloningandcharacterizationofanovelp70S6kinase,p70S6kinasebetacontainingaproline-richregion.J.Biol.Chem.273:30061-30064,1998.
2.Saitoh,M.etal:Cloningandcharacterizationofp70(S6K-beta)definesanovelfamilyofp70S6kinases.Biochem.Biophys.Res.Commun.253:470-476,1998.
2.Saitoh,M.etal:Cloningandcharacterizationofp70(S6K-beta)definesanovelfamilyofp70S6kinases.Biochem.Biophys.Res.Commun.253:470-476,1998.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
