Bpsbioscience/NEK3, GST-tag/40140/10 µg
商品编号：
40140
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:86kDa
Genbank#:NM_002498
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
NEK3isamemberoftheNEKfamilyofproteinkinasesthatsharehighaminoacidhomologywithNIMA(neverinmitosisgenea).NEK3mRNAisdetectedinalltheproliferatingcelllineswiththeamountnotchangingduringthecellcycle(1).DuringProlactinreceptorsignaling,VAV2isphosphorylatedandactivatedbyNEK3.OverexpressionofNEK3inChinesehamsterovarycellsincreasescytoskeletalre-organizationinresponsetoProlactinwhiledownregulationofNEK3expressionbysiRNAblocktheseeffects(2).ProlactinalsostimulatesinteractionbetweenNEK3andpaxillinleADIngtoincreasedpaxillinphosphorylation,Analysisofbreasttissuemicroarraysshowasignificantup-regulationofNEK3expressioninmalignantversusnormalspecimens.
Description:
RecombinanthumanNEK3,fulllength,withN-terminalGST-tag,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
HSPK36,MGC29949
Purity:
≥75%
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Kimura,M.etal:MolecularcloningandcharacterizationofthehumanNIMA-relatedproteinkinase3gene(NEK3).CytogenetCellGenet.2001;95(3-4):177-82.
2.Miller,S.L.etal:Nek3kinaseregulatesprolactin-mediatedcytoskeletalreorganizationandmotilityofbreastcancercells.Oncogene.2007Jul12;26(32):4668-78.
2.Miller,S.L.etal:Nek3kinaseregulatesprolactin-mediatedcytoskeletalreorganizationandmotilityofbreastcancercells.Oncogene.2007Jul12;26(32):4668-78.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
