Bpsbioscience/NEK2/40009/10 µg
商品编号：
40009
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 53 kDa
Genbank #: NM_002497
Tag(s): N-terminal His-tag
a.a: full length
Description:
Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
NEK2, NIMA-related kinase 2, Never In Mitosis-Related Kinase 2
Specific Activity:
100 U/mg
Unit Definition:
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.
Assay Conditions:
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 0.3 mg/ml MBP substrate, and 0.2 µg/ml NEK2.
Formulation:
25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween-20, 40% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fletcher, L. et al., Biochim. Biophys. Acta 1744(2):89-92 (2005).
2. Fardilha, M. et al., Ann. N.Y. Acad. Sci. 1030:468-478 (2004).
Application Reference:
Nek2 phosphorylates and stabilizes β-catenin at mitotic centrosomes downstream of Plk1 (2014)
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
