Bpsbioscience/Mouse Sphingosine kinase 1a, His-tag/40613/10 µg
商品编号：
40613
品牌：
bpsbioscience
市场价：
¥8300.00
美元价：
4980.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:Sf9insectcells
MW:47.1kDa
Genbank#:AF415213
Tag(s):N-terminalHis-tag
a.a:fulllength
Description:
MouseSphingosinekinase1(GenBankAccessionNo.AF415213),fulllengthwithN-terminalHis-tag,MW=47.1kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
SphK1,sphingosinekinase1,SPK1
SpecificActivity:
20U/µg
UnitDefinition:
Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1Pperminuteat37°C.
AssayConditions:
SphingosinekinaseactivitywasdeterminedusingD-erythro-sphingosineandATPassubstrates.Assaycondition:50mMHEPES,pH7.4,150mMNaCl,5mMMgCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP,4µMD-erythrosphingosine,and0.1-0.5µg/mlsphingosinekinase1at37°Cfor30min.ATPreductionisdetectedusingKinase-Glo®LuminescentKinaseAssayPlatform(PromegaCorporation,MADIson,WI).
Formulation:
25mMTris-HCl,pH8.0,100mMNaCl,0.05%Tween-20,50%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Delon,C.,etal.,J.Biol.Chem279(43)44763-44774(2004)
2.Taha,TAetal.,FASEBJ.20(3):482-4(2005)
2.Taha,TAetal.,FASEBJ.20(3):482-4(2005)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Lipids
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
