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当前位置: 首页 > 产品中心 > Routine_cell_strain > Bpsbioscience/正常人外周血单个核细胞,冷冻/79059/3000万细胞
商品详细Bpsbioscience/正常人外周血单个核细胞,冷冻/79059/3000万细胞
Bpsbioscience/正常人外周血单个核细胞,冷冻/79059/3000万细胞
Bpsbioscience/正常人外周血单个核细胞,冷冻/79059/3000万细胞
商品编号: 79059
品牌: bpsbioscience
市场价: ¥3500.00
美元价: 2100.00
产地: 美国(厂家直采)
公司:
产品分类: 常规细胞株
公司分类: Routine_cell_strain
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description:
Cryopreservedvial(30x10^6cells)offreshlyisolatedprimaryhumanperipheralbloodmononuclearcells(PBMCs)fromahealthydonor,isolatedfromleukapheresis/apheresissamplesusingFicollgrADIent.Afterisolation,thePBMCswerestainedtoidentifysubpopulationsandevaluatedforviABIlitybyflowcytometry.CellswerecryopreservedinFBSwith10%DMSOatacontrolledrate.
GrowthMedia:
10%FBSinIsocove’sModifiedDulbecco’sMedium(IMDM;Lonza,#12-722)orLymphocyteGrowthMedium3(LGM-3;Lonza,#CC-3211)
Instructionsforuse:
Preparea50mlconicaltubewith10mlofpre-warmedmedium(10%FBSinIMDMorLGM).Quicklythawcellsina37°Cwaterbathwithconstantandslowagitation.Itisimportanttoworkquicklyinthefollowingstepstoensurehighcellviabilityandrecovery.Cleantheoutsideofthevialwith70%ethanolandimmediatelytransfertheentirecontentofthetubeintothemedium.GentlyswirlthetubeandcentrifugethecellsUSPensionat300xgfor10minutesatroomtemperature.

CarefullyremovethesupernatantwithaPipettewithoutdisturbingthepellet.Gentlyresuspendthecellpelletin15-20mLofwarmmedium.Centrifugethecellsuspensionat300xgfor10minutesatroomtemperature.Carefullyremovethesupernatantwithapipettewithoutdisturbingthepellet.Gentlyresuspendthecellpelletinwarmmediumandmixbygentlyflickingthetube.NOTE:Upto30%ofcelllosscanbeexpectedduringwashingsteps.Cellsarenowreadyforuseindownstreamapplications.

Storage/Stability:
Storecellsat-135˚Corcolder.Thawedcellsshouldbeusedimmediatelyfordownstreamapplications.Astheseareprimarycells,wedonotrecommendmaintainingthesecellsinculture.
ScientificCategory:
Immunotherapy
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。