Bpsbioscience/NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line/60651/2 vials-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line/60651/2 vials

Bpsbioscience/NF-κB Luciferase Reporter (Luc) - Jurkat Recombinant Cell Line/60651/2 vials

商品编号： 60651

品牌： bpsbioscience

市场价： ¥30000.00

美元价： 18000.00

产地：美国(厂家直采)

公司：

产品分类：常规细胞株

公司分类： Routine_cell_strain

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description:

NF-ĸBluciferasereporterconstructisstablyintegratedintothegenomeofJurkatT-cells.Thefireflyluciferasegeneiscontrolledby4copiesofNF-kBresponseelementlocatedupstreamoftheTATApromoter.Followingactivationbystimulants,endogenousNF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasegene.

MaterialsRequiredButNotProvided:

TNFα(Sigma,#T0157-10UG)
PMA(LCLaboratories,#P1680)
Ionomycin(Sigma,#I3909)
AssayMedium:ThawMedium2(BPSCat.#60184)
96-welltissueculturetreatedwhiteclear-bottomassayplate(Corning#3610)
ONE-Stepluciferaseassaysystem(BPSCat.#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer

Synonym(s):

nfkb

HostCellline:

HumanAcuteT-CellLeukemicCellLine(CloneE61).SUSPensioncells.

MycoplasmaTesting:

ThiscelllinehasbeenscreenedusingtheMycoAlert™MycoplasmaDetectionKit(Cat.No.LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Cat.No.LT07-518)wasusedasapositivecontrol.

Format:

Eachvialcontains~2x10^6cellsin1mLof10%DMSOinFBS.

Storage/StABIlity:

Storeinliquidnitrogenimmediatelyuponreceipt.

Application(s):

TheNF-ĸB-luciferase/JurkatcelllineissuitableformonitoringtheactivityofNF-ĸBsignalinginresponsetostimulants,andestablishingcell-basedscreensforinhibitorsthattargetspecificNF-ĸB-stimulatingmolecules.

Reference(s):

1.BornerCandKrausJ(2013)InhibitionofNF-kBbyOpioidsinTcells.JImmunology.191.

2.MoriN.et.al.(2002)Bay11-7082inhibitstranscriptionfactorNF-kBandinducesapoptosisofHTLV-1-infectedTcelllinesandprimaryadultT-cellleukemiacells.Blood.100:1828-1834

3.TakadaY,KobayashiY,AggarawalBB(2005)EvodiamineabolishesconstitutiveandinducIBLeNF-kappaBactivationbyinhibitingIkappaBalphakinaseactivation,therebysuppressingNF-kappaB-regulatedantiapoptoticandmetastaticgeneexpression,up-regulatingapopotissandinhibitinginvasion.JBiolChem.280:17203

Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

ScientificCategory:

Immunotherapy

ProductType:

CellLine

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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