NETisamonoaminetransporterresponsIBLeforthesodium-chloride(Na+/Cl−)-dependentreuptakeofextracellularnorepinephrine(NE),alsoknownasnoradrenaline.NETcanalsoreuptakeextracellulardopamine(DA).Thereuptakeofthesetwoneurotransmittersisessentialinregulatingconcentrationsinthesynapticcleft.NETs,alongwiththeothermonoaminetransporters,playakeyroleindepressionandneurodegenerativediseases(NDD)suchasAlzheimer"sandParkinson"sdisease.NETisthetargetofmanyantidepressantsandrecreationaldrugs.Inaddition,anoverabundanceofNETisassociatedwithADHD.
ThawMedium3(BPSCat.#60186):Ham’sF-12medium(Hyclone#SH30526.01)supplementedwith10%FBS(Lifetechnologies#26140-079),1%Penicillin/Streptomycin(HycloneSV30010.01)
CompleteGrowthMedium:ThawMedium3(BPSCat.#60186)plus500μg/mlofHygromycinB(LifeTechnologies#10687-010)toensuretherecombinantexpressionismaintained.Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmedium.
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FLIPRvalidationdataisavailableuponrequest.
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