Bpsbioscience/mTOR/Raptor/mLST8 Complex, FLAG-tag, His-tag (Human)/40300/10 µg
商品编号：
40300
品牌：
bpsbioscience
市场价：
¥8500.00
美元价：
5100.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:324kDa(complex)
Genbank#:NM_004958(mTOR),NM_022372(MLST8),NM_020761(Raptor)
Tag(s):N-terminalFLAG-tag(mTOR),N-terminalHis-tag(MLST8),N-terminalHis-tag(Raptor)
a.a:mTOR1362–end,MLST82–326(end),Raptor2–1335(end)
Description:
ComplexofhumanmTOR,alsoknownasFRAP1(GenBankAccessionNo.NM_004958),a.a.1362-end,withN-terminalFLAG-tag,MW=137kDa,humanMLST8(GenBankAccessionNo.NM_022372),a.a.2-326(end)withN-terminalHis-tag,MW=37kDa,andhumanRaptor(GenBankAccessionNo.NM_020761),a.a.2-1335(end)withN-terminalHistag,MW=150kDa,co-expressedinaBaculovirus-infectedSf9cellexpressionsystem.
UniProt
P42345(mTOR),Q9BVC4(MLST8),Q8N122(Raptor)
Synonym(s):
mTOR,mechaNISTictargetofrapamycin,FRAP,FKBP-RapamycinAssociatedProtein,MammalianTargetOfRapamycin,raptor,regulatoryassociatedproteinofMTOR,Mip1,MLST8,MammalianLethalWithSEC13Protein8,GBL,Gable,POP3,LST8
SpecificActivity:
7.1pmol/min/µg
AssayConditions:
Enzymereactionisconductedinabuffercontaining50mMHEPES(pH7.5),10mMMgCl2,1mMEGTA,200µMATP,0.01%Brij-35and2µMsubstrate(InvitrogenZlyteSer/Thrpeptide11)atroomtemperaturefor1hour.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,10%glycerol,and0.18mg/mlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Toral-Barzaetal.,(2005)Biochem.Biophys.Res.Commun.24,332(1):304-10.
2.Rubio-ViqueiraandHidalgo,M.(2006)Adv.Exp.Med.Biol.587:309-327.
ApplicationReference(s):
1.mTORsignalingregulatesnucleolartargetingoftheSUMO-specificisopeptidaseSENP3(2014)
2.Themechanistictargetforrapamycinpathwayisrelatedtothephosphorylationscoreforestrogenreceptor-αinhumanbreasttumorsinvivo(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine,Immunotherapy
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
