Bpsbioscience/IL-2-荧光素酶报告(Luc)-Jurkat细胞系/60481/2小瓶
商品编号:
60481
品牌:
bpsbioscience
市场价:
¥115500.00
美元价:
69300.00
产品分类:
常规细胞株
公司分类:
Routine_cell_strain
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
Interleukin-2(IL-2)isakeycytokineimportantforproliferationanddifferentiationofTcellsintoeffectorTcellsthroughinteractionwiththeIL-2receptor.
Description:
HumanIL-2reporterconstructisstablyintegratedintothegenomeofJurkatT-cells.ThefireflyluciferasegeneiscontrolledbyahumanIL-2promoter.
MaterialsRequiredButNotProvided:
ThawMedium2(BPSCat.#60184)
ONE-StepLuciferaseDetectionReagent(BPSCat.#60690-1)
PMA(Phorbol12-Myristate13-Acetate,Fisher,#BP685-1),1mg/mlstockinDMSO
Ionomycin(Fisher,#BP25271,1mg/mlstockinDMSO
Anti-humanCD3antibody(BPSCat.#71274)
Anti-CD28antibody(Clone28.2,BDBiosciences,#555725)
TCRActivator/RajiRecombinantCells(BPSCat.#60556)
HostCellline:
Jurkat
MycoplasmaTesting:
ThiscelllinehasbeenscreenedusingtheMycoAlert™MycoplasmaDetectionKit(Cat.No.LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Cat.No.LT07-518)wasusedasapositivecontrol.
Format:
Eachvialcontains~2x10^6cellsin1mlof10%DMSOinFBS.
Storage/StABIlity:
Storeinliquidnitrogenimmediatelyuponreceipt.
Application(s):
TheIL-2-luciferaseJurkatreportercelllineissuitableformonitoringthetranscriptionactivityofIL-2inresponsetostimulants,andestablishingcell-basedscreensforinhibitorsthattargetspecificIL-2stimulatingmolecules.
Reference(s):
1.WeaverJRet.al.(2007)Characterizationofthesequenceandarchitecturalconstraintsoftheregulatoryandcoreregionsofthehumaninterleukin-2promoter.Mol.Immunol.44:2813-2819.
2.HughesCCWandPoberJS(1996)TranscriptionalRegulationoftheInterleukin-2GeneinNormalHumanPeripheralBloodTCells.J.Biol.Chem.271:5369-5377.
Notes:
LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
ScientificCategory:
Immunotherapy
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。