Bpsbioscience/小鼠干细胞因子/90236-B/10µg
商品编号：
90236-B
品牌：
bpsbioscience
市场价：
¥5200.00
美元价：
3120.00
产品分类：
常规细胞株
公司分类：
Routine_cell_strain
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:E.coli
MW:23kDa
Genbank#:P20826
a.a:26–189
Background:
SCFisastromalcell-derivedcytokinesynthesizedbyfibroblastsandothercelltypes.SCFpromotesproliferationandearlydifferentiationofcellsatthelevelofmultipotentialstemcells.IthasbeensuggestedthatSCFisessentialforoptimalproductionofvarioushematopoieticlineages,mainlybecauseofitsABIlitytopreventapoptosiswhenitco-stimulateswithothercytokines.ThereceptorforSCF,designatedSCFR(CD117),istheoncogenedesignatedasKIT.TheBIOLOGicalactivitiesofSCFaresynergisedconsiderablybycolonystimulatingfactorsGM-CSFandG-CSF,andalsobyIL-7,Epoandsomeothergrowthanddifferentiationfactors.IncombinationwithIL-7,SCFstimulatestheproliferationofpre-B-cells.SCFisalsoapotentchemoattractantforcells,forexample,mastcells,expressingthekitreceptor.OneresponsetoSCFinthesecellsisacharacteristicrearrangementoftheactinfilamentsoftheCytoskeleton.
Description:
RecombinantSCF(KitLigand)isadisulfide-linkedmonomerproteinconsistingof165aminoacidresidues,andduetoglycosylationmigratesasanapproximately23kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingmurineSCFmaturechainwasexpressedinE.coli.
UniProt
P20826
Synonym(s):
KitLigand,SCF,StemCellFactor,SteelFactor,MGF,andMastCellGrowthFactor,KITL,CD117L
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1cellsis<2.0ng/ml,correspondingtoaspecificactivityof>5x105units/mg.
Formulation:
Lyophilizedfroma0.2µmfilteredsolutionin20mMTris,5%trehalose,pH7.5.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/Stability:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.BedellMA,etal.JAndrol.2004Mar-Apr,25(2):188-99.
2.HeissigB,etal.ThrombHaemost.2003Oct,90(4):570-6.
3.Wehrle-HallerB.PigmentCellRes.2003Jun,16(3):287-96.
2.HeissigB,etal.ThrombHaemost.2003Oct,90(4):570-6.
3.Wehrle-HallerB.PigmentCellRes.2003Jun,16(3):287-96.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
KEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
