Bpsbioscience/小鼠白细胞介素-4/90194-A/2µg
商品编号：
90194-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:E.coli
MW:14kDa
Genbank#:P07750
a.a:21–140
Background:
Interleukin-4isproducedmainlybyasubpopulationofactivatedT-cells(Th2)thataretheBIOLOGicallymostactivehelpercellsforB-cellsandthatalsosecreteIL-5andIL-6.ThebiologicalactivitiesofIL-4aremediatedbyaspecificreceptor.TheextracellulardomainoftheIL-4receptorisrelatedtothereceptorsforEPO,IL-6,andthebetachainoftheIL2receptor(CD124).TwotypesofIL-4receptor(IL-4R)exist:thetype1receptorisaheterodimerconsistingofCD132andIL-4R-alpha.Thetype2receptorisaheterodimerconsistingofIL-4RalphaandIL-13Ralpha1.IL-4enhancesexpressionofMHCclass2antigensonB-cells.ItcanpromotetheircapacitytorespondtootherB-cellstimuliandtopresentantigensforT-cells.PretreatmentofmacrophageswithIL-4preventstheproductionofIL1,TNF-alphaandprostaglandinsinresponsetoactivationofthecellsbybacterialendotoxinsorIFN-gamma.IL-4synergiseswithEPOandG-CSFinthegenerationofcoloniescontaininggranulocytesorerythroidProgenitorcellsinacolonyformationassay.
Description:
RecombinantmouseInterleukin-4isadisulfide-linkedmonomerproteinconsistingof121aminoacidresidues,migratesasanapproximately14kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingmouseInterleukin-4maturechainwasexpressedinE.coli.
UniProt
P07750
Synonym(s):
IL-4,interleukin4,BCGF1,BcellGrowthFactor-1,IL4
Purity:
≥96%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1cellsis≤0.4ng/ml,correspondingtoaspecificactivityof≥2x106units/mg.
Formulation:
Lyophilizedfroma0.2µmfilteredPBSsolution,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.TachdjianR,etal.J.Exp.Med.,Sep2009,206:2191-2204.
2.Yu,M.,etal.JBiolChem.2009Nov20,284(47):32968-79..
3.EnnaciriJ,GirardD.JImmunol.2009Oct15,183(8):5261-9
2.Yu,M.,etal.JBiolChem.2009Nov20,284(47):32968-79..
3.EnnaciriJ,GirardD.JImmunol.2009Oct15,183(8):5261-9
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
HIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
