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当前位置: 首页 > 产品中心 > cytokine > Bpsbioscience/小鼠白细胞介素-10/90174-B/10µg
商品详细Bpsbioscience/小鼠白细胞介素-10/90174-B/10µg
Bpsbioscience/小鼠白细胞介素-10/90174-B/10µg
Bpsbioscience/小鼠白细胞介素-10/90174-B/10µg
商品编号: 90174-B
品牌: bpsbioscience
市场价: ¥4100.00
美元价: 2460.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:E.coli
MW:19kDa
Genbank#:P18893
a.a:19–178
Background:
IL-10isproducedbymouseTh2-cellsfollowingtheirstimulationbylectins.ThemainsourceforB-cellderivedIL-10inmiceareLy1B-cellsthatexpressCD5(Ly1)andCD11.InhumansIL-10isproducedbyactivatedCD8(+)peripheralbloodT-cells,byT-helperCD4(+)T-cellclonesafterbothantigen-specificandpolyclonalactivation,byB-celllymphomas,andbymonocytesfollowingcellactivationbybacteriallipopolysaccharidesandmastcells.IL-10isahomodimericproteinwithsubunitshavingalengthof160aminoacids.HumanInterleukin-10shows73%aminoacidhomologywithmouseInterleukin-10.ThemurineIL-10receptorhasbeencloned.Thisreceptorisaproteinofapproximately110kDathatbindsmurineIL-10specifically.ThisreceptorisstructurallyrelatedtoreceptorsforIFN.TheCDnomenclatureforthisreceptorisCDw210.IL-10inhibitsthesynthesisofanumberofcytokinessuchasIFN-gamma,IL-2andTNF-betainTh1T-helpersubpopulationsofT-cellsbutnotofTh2T-helpercells.ThisactivityisantagonizedbyIL-4.TheinhibitoryeffectonIFN-gammaproductionisindirectandappearstobetheresultofasuppressionofIL-12synthesisbyaccessorycells.Inthehumansystem,IL-10isproducedby,anddown-regulatesthefunctionofTh1andTh2cells.
Description:
RecombinantMouseIL-10isadisulfide-linkedmonomerproteinconsistingof161aminoacidresidues,andmigratesasanapproximately19kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingmouseInterleukin-10maturechainwasexpressedinE.coli.
UniProt
P18893
Synonym(s):
IL-10,Interleukin10,CytokineSynthesisInhibitoryFactor,CSIF,TGIF,IL10
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50wasdeterminedbythedose-dependent(withIL-4)proliferationofmurineMC/9cells,andwasfoundtobe<2ng/ml,correspondingtoaspecificactivityof>5x105units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredPBS,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:

Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.

Reference(s):
1.J.Immunol.,Oct2009,183:4475-4482.
2.J.Immunol.,Oct2009,183:4619-4627.
3.J.Exp.Med.,Sep2009,206:2235-2251.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLKCENKSKAVEQVKSDFNKLEDQGVYKAMNEFDIFINCIEAYMMIKMKS
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。