Bpsbioscience/小鼠粒细胞-巨噬细胞集落刺激因子/90146-A/5µg
商品编号：
90146-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:E.coli
MW:14kDa
Genbank#:P01587
a.a:18–141
Background:
GM-CSFisamonomericproteinof127aminoacidswithtwoglycosylationsites.Theproteinissynthesizedasaprecursorof144aminoacids,whichincludedahydrophobicsecretorysignalsequenceattheN-terminalend.ThesugarmoietyisnotrequiredforthefullspectrumofBIOLOGicalactivities.Non-glycosylatedandglycosylatedGM-CSFshowthesameactivitiesinvitro.FullyglycosylatedGM-CSFisbiologicallymoreactiveinvivothanthenon-glycosylatedprotein.ThisproteinissecretedtogetherwithotherfactorsbyT-cellsandmacrophagesfollowingcellactivationbyantigensormitogens.Approximately90%ofthesecretedcolonystimulatingactivitiesareduetoGM-CSF.
Description:
RecombinantmouseGM-CSFisadisulfide-linkedmonomerprotein,consistingof125aminoacidsandmigratesasanapproximately14kDaproteinunderreducingconditions.OptimizedDNAsequenceencodingmurineGranulocyteMacrophage-ColonyStimulatingFactormaturechainwasexpressedinE.coli.
UniProt
P01587
Synonym(s):
GM-CSF,CSF2,GranulocyteMacrophage-ColonyStimulatingFactor,ColonyStimulatingFactor-2,Molgramostin,Sargramostim,CSF
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofmurineFDC-P1cellsis≤0.1ng/ml,correspondingtoaspecificactivityof≥3x106units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredPBSsolution,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Hiasa,M.,etal.BloodNovember12,2009vol.114(20):4517-4526.
2.Zhu,S.M.,etal.J.Exp.Med.206(10):2141-2149.
3.Chin,P.Y.,etal.Hum.Reprod.(2009)24(12):2997-3009.
2.Zhu,S.M.,etal.J.Exp.Med.206(10):2141-2149.
3.Chin,P.Y.,etal.Hum.Reprod.(2009)24(12):2997-3009.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPGQK
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
