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Bpsbioscience/PRMT6 (HRMT1L6) Homogeneous Assay Kit/52056/384 reactions
Bpsbioscience/PRMT6 (HRMT1L6) Homogeneous Assay Kit/52056/384 reactions
商品编号：
52056
品牌：
bpsbioscience
市场价：
¥28300.00
美元价：
16980.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
PRMT6isahistonemethyltransferasethatexhibitsmethylationactivitytowardH3-R2andH4-R3.
Description:
ThePRMT6HomogeneousAssayKitisdesignedtomeasuretheactivityofPRMT6forscreeningandprofilingapplications,usingAlphaLISA®technology.ThekeytothePRMT6HomogeneousAssayKitisahighlyspecificantibodythatrecognizesmethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,asamplecontainingPRMT6enzymeisincubatedwiththebiotinylatedsubstrateforonehour.Next,acceptorbeadsandprimaryantibodyareadded,thendonorbeads,followedbyreADIngtheAlphacounts.
Synonym(s):
PRMT6,HRMT1L6
SuppliedAs:
ThePRMT6HomogeneousAssayKitcomesinaconvenientAlphaLISA®format,withbiotinylatedhistoneH4peptidesubstrate,primaryantibody,methylationassaybuffer,andpurifiedPRMT6for384enzymereactions.
Contraindications:
AvoidgreenandbluedyesthatabsorblightintheAlphascreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphascreen®assays.
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Yang,Y.,Bedford,M.T.2013.NatRevCancer.13(1):37-50.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
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品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
