Bpsbioscience/PRMT3, GST-tag/51043/50 µg
商品编号：
51043
品牌：
bpsbioscience
市场价：
¥6600.00
美元价：
3960.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 86 kDa
Genbank #: NM_005788
Tag(s): N-terminal GST-tag
a.a: 2–end
Background:
PRMT3 is a protein arginine methyltransferase that catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins. 40S ribosomal protein S2 is a physiological substrate of PRMT3 and PRMT3 regulates ribosome biosynthesis at a stage beyond pre-rRNA processing. DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene that can significantly inhibits PRMT3 methylation of cellular substrates. Modulation of post-translational methylation may be an important mechanism through which DAL-1/4.1B affects tumor cell growth.
Description:
Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system.
UniProt
Q8WUV3
Synonym(s):
PRMT3, HRMT1L3
Specific Activity:
490 pmol/min/µg
Assay Conditions:
50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-10 ng methyltransferase PRMT3) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Choi S. et al., Biochim Biophys Acta. 2008 Sep,1780, 9, :1062-9.
2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.
2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
