Bpsbioscience/PRMT1, GST-tag (Sf9-derived)/51041/20 µg
商品编号：
51041
品牌：
bpsbioscience
市场价：
¥6600.00
美元价：
3960.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 68 kDa
Genbank #: NM_001536
Tag(s): N-terminal GST-tag
a.a: 2–end
Background:
PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Description:
Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2-end, with N-terminal GST-tag, MW= 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q99873
Synonym(s):
PRMT1, HRMT1L2, IR1B4, HCP1
Specific Activity:
6.2 pmol/min/µg
Assay Conditions:
50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT1) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation:
40 mM Tris pH 8.0, 300 mM NaCl, 20% glycerol and 1 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Sun X.J., et al., J Biol Chem. 280, 42, 35261-35271, 2005.
2. Rega S. et al., Mol Cell Neurosci. 18, 1, 68-79, 2001.
Application Reference(s):
1. A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014)
2. Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
