Bpsbioscience/Jak2 (JH1 domain), His-tag/40450/10 µg
商品编号:
40450
品牌:
bpsbioscience
市场价:
¥7100.00
美元价:
4260.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 42.5 kDa
Genbank #: NM_004972
Tag(s): N-terminal His-tag
a.a: 808–end
Description:
Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system.
Synonym(s):
JAK2, Janus kinase-2, Just Another Kinase 2
Specific Activity:
118 pmol/min/μg.
Assay Conditions:
50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Cytoplasmic Tyr
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
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