StableRecombinantHEK293IDO1celllineexpressingtetracycline-inducIBLehumanindoleamine2,3-dioxygenase(IDO1)GenbankaccessionnumberNM_002164.

• ThawMedium1(BPSCat.#60187)
• Geneticin(Sigma,#G8168)and BlasticidinS(Sigma(#15205)
• Doxycycline(MPBiomedicals#0219504401) 
• PBS 
• 0.05%TrypsinEDTA 
• 6.1NTrichloroaceticacid(Sigma#T0699) 
• Aceticacid(Sigma#320099)  
•IDO1  Cellular  Activity  QuickDetect™Supplements  (BPS  Cat.  #62000). Note:otherformulationscanbeused,butsignificantoptimizationmayberequired. 

1.Yue,E.,etal.,J.Med.Chem.2009;52:7364–7367.
2.Liu,X.,etal.,Blood.2010;115(17):3520-3530.

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