Bpsbioscience/IDO1 Fluorogenic Inhibitor Screening Assay Kit/72037/96 reactions-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/IDO1 Fluorogenic Inhibitor Screening Assay Kit/72037/96 reactions

Bpsbioscience/IDO1 Fluorogenic Inhibitor Screening Assay Kit/72037/96 reactions

商品编号： 72037

品牌： bpsbioscience

市场价： ¥14900.00

美元价： 8940.00

产地：美国(厂家直采)

公司：

产品分类：其它检测试剂盒

公司分类： Other_kits

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商品介绍

Background:

L-tryptophan(L-Trp)isanessentialaminoacidnecessaryforproteinsynthesisinmammaliancellsandtheL-Trptokynurenine(Kyn)pathwayisfirmlyestablishedasakeyregulatorofinnateandadaptiveimmunity.CatabolismofL-TrptoKynmaintainsanimmunosuppressivemicroenvironmentbystarvingimmunecellsofL-TrpandreleasingdegradationproductsofL-Trpthathaveimmunosuppressivefunctions.Indoleamine2,3-dioxygenases(IDO1&IDO2),twooftheratelimitingenzymesinthispathway,areupregulatedinmanytumors,providingcancercellswithanavenueforimmuneevasion.

Description:

TheIDO1FluorogenicInhibitorScreeningAssayKitisdesignedtomeasureenzymeinhibitionofindoleamine2,3-dioxygenase1(IDO1).TheIDO1FluorogenicInhibitorScreeningAssayKitissimpletouseanddetectsfluorescenceatlongwavelengths,whichminimizespotentialerrorsduetocompoundinterference.Intheassay,theinhibitorandenzymeareaddedtoasamplecontainingL-Trpsubstrate.Aftera1hourincubationatroomtemperature,thefluorescencesolutionisaddedandincubatedat37oCforfourhours.ActivityismeasuredbyreADIngsamplefluorescenceatl=510nmfollowingexcitationofthereactionproductatl=400nm.

Synonym(s):

Indoleamine2,3-dioxygenase1,IDO

SuppliedAs:

Thekitcomesinaconvenientformat,withenoughreactionsolutionandenzymetoperformatotalof96reactions.

Contraindications:

DMSO>0.5%,strongacidsorbases,ionicdetergents,highsalt.Warning:theFluorescenceSolutioncontainsacomponentthatisknowntobeaskinandeyeirritant,usecautionandappropriatepersonalprotectiveequipmentwhenhandlingthiscomponent.

Format:

COMPONENTS:

Instructionsforuse:

Seeassaykitdatasheetfordetailedprotocol

Storage/StABIlity:

Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival

Application(s):

UsefulforthestudyofIDO1enzymology,inhibitorscreening,andselectivityprofiling.

Reference(s):

Seegers,N.,etal.J.Biomol.Screen.2014.19(9):1266-74.

Warning(s):

Avoidfreeze/thawcycles.

TheFluorescenceSolutioncontainsacomponentthatisknowntobeaskinandeyeirritant,usecautionandappropriatepersonalprotectiveequipmentwhenhandlingthiscomponent.

ScientificCategory:

Immunotherapy

ProductType:

AssayKit

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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