TheIDO1CellularActivityQuickDetect™SupplementsaredesignedtocomplementthehIDO1-HEK293RecombinantCellLine(BPSCat.#60532)orotherIDO1-expressingcelllines.ThiskitcontainsthecellculturemediumsupplementsnecessaryforactivationofIDO1andfortheanalysisanddetectionofIdoleamine2,3dioxygenase1(IDO1)-catalyzedconversionofL-tryptophan(L-Trp)toKynurenine(Kyn).Thesupplementsandthedetectionreagents,whenusedasdescribed,allowforindirectmeasurementofKynlevelsbyanalyzingabsorptionat480nm.
hIDO1-HEK293RecombinantCellLine(BPSCat.#60532)orotherIDO1-expressingcelllineandappropriatecellculturemedium
INCB024360(BPSCat.#27339)orotherIDO1inhibitor
6.1N(concentrated)trichloroaceticacid*
17.4N(concentrated)glacialaceticacid*
*Note:bothtrichloroaceticacidandaceticacidarestronglycorrosiveacids;pleaseuseglovesandappropriateprotectiveclothing.
1.Liu,X.,etal.,Blood.2010;115(17):3520-3530.
Contactusforpricing.
LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
