Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials

Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials

商品编号： 79310

品牌： bpsbioscience

市场价： ¥176000.00

美元价： 105600.00

产地：美国(厂家直采)

公司：

产品分类：常规细胞株

公司分类： Routine_cell_strain

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Background:

HVEMisabidirectionalswitchregulatingT-cellactivationinacostimulatoryorcoinhibitoryfashionwhoseoutcomedependsonthebindingpartner.HVEMcanactasbothreceptorandligand,thebindingofendogenousligandLIGHToragoNISTantibodiestoHVEMdeliversacostimulatorysignal;whereasthebindingofHVEMtoBTLA(IgSF)orCD160onEffectorTcellsdeliversacoinhibitorysignal.LIGHT/HVEMaxisareco-stimulatoryimmunecheckpointmoleculesextensivelystudiedforcancerimmunotherapy;LIGHT,eitherinthesolublemonomerformorhomotrimerexpressedonthecellsurface,canactivateNF-kBthroughbindingtoHVEM;wehaveshownthatLIGHTexpressingcellsaremorepotentandefficaciousthanthesolubleLIGHTforactivatingHVEMexpressedonTcellsurface.

Description:

RecombinantclonalstableJurkatTcelllineexpressingfireflyluciferasegeneunderthecontrolof4copiesofNF-κBresponseelementswithconstitutiveexpressionofhumanHVEM(HerpesVirusEntryMediator,TumorNecrosisFactorReceptorSuperfamilyMember14,TNFRSF14,CD270,GenBankAccessionNo.NM_003820).FollowingactivationbyhumanHVEMligandLIGHT,NF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasereportergene.

MaterialsRequiredButNotProvided:

AssayMedium:ThawMedium2(BPSCat.#60184)
LIGHT,His-Tag(Human)(BPSCat.#71266)
LIGHT-CHORecombinantCellLine(BPSCat.#79262)
96-welltissueculture-treatedwhiteclear-bottomassayplate
One-Stepluciferaseassaysystem(BPSbioscience#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer

Synonym(s):

HVEM,TNFRSF14,CD270,HVEA,TR2,LIGHTR

SuppliedAs:

Eachvialcontains~2x10^6cellsin1mlof10%DMSO.

HostCellline:

JurkatTcell

MycoplasmaTesting:

ThiscelllinehasbeenscreenedusingtheMycoAlert™MycoplasmaDetectionKit(Lonza,#LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Lonza,#LT07-518)wasusedasapositivecontrol.

GrowthMedia:

ThawMedium2(BPSCat.#60184):RPMI1640medium(LifeTechnologies#A10491-01)supplementedwith10%FBS(LifeTechnologies#26140-079),1%Penicillin/Streptomycin(Hyclone#SV30010.01)

CompleteGrowthMedium:ThawMedium2(BPSCat.#60184)plus1mg/mlG418(ThermoFisher,Cat.#11811031)and200μg/mlofHygromycinB(Hyclone#SV30070.01)

Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmedium(ThawMedium2withG418andHygromycinB).

Instructionsforuse:

Seedatasheetfordetailedculturingandassayprotocol.

Storage/StABIlity:

Storeinliquidnitrogenimmediatelyuponreceipt.

Application(s):

•ScreenforagonistsorantagonistsofLIGHT-HVEMsignalinginaphysiologicalrelevantcellularcontext

•CharacterizeTcell-mediatedimmuneresponsesofHVEManditsinteractionswithLIGHT

•Screenco-stimulatoryimmunecheckpointmolecules

Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermittheuseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

Warning(s):

Avoidfreeze/thawcycles.

ScientificCategory:

Immunotherapy

ProductType:

CellLine

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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