4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Routine_cell_strain > Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials
商品详细Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials
Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials
Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials
商品编号: 79310
品牌: bpsbioscience
市场价: ¥176000.00
美元价: 105600.00
产地: 美国(厂家直采)
公司:
产品分类: 常规细胞株
公司分类: Routine_cell_strain
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Background:
HVEMisabidirectionalswitchregulatingT-cellactivationinacostimulatoryorcoinhibitoryfashionwhoseoutcomedependsonthebindingpartner.HVEMcanactasbothreceptorandligand,thebindingofendogenousligandLIGHToragoNISTantibodiestoHVEMdeliversacostimulatorysignal;whereasthebindingofHVEMtoBTLA(IgSF)orCD160onEffectorTcellsdeliversacoinhibitorysignal.LIGHT/HVEMaxisareco-stimulatoryimmunecheckpointmoleculesextensivelystudiedforcancerimmunotherapy;LIGHT,eitherinthesolublemonomerformorhomotrimerexpressedonthecellsurface,canactivateNF-kBthroughbindingtoHVEM;wehaveshownthatLIGHTexpressingcellsaremorepotentandefficaciousthanthesolubleLIGHTforactivatingHVEMexpressedonTcellsurface.
Description:

RecombinantclonalstableJurkatTcelllineexpressingfireflyluciferasegeneunderthecontrolof4copiesofNF-κBresponseelementswithconstitutiveexpressionofhumanHVEM(HerpesVirusEntryMediator,TumorNecrosisFactorReceptorSuperfamilyMember14,TNFRSF14,CD270,GenBankAccessionNo.NM_003820).FollowingactivationbyhumanHVEMligandLIGHT,NF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasereportergene.

MaterialsRequiredButNotProvided:
  • AssayMedium:ThawMedium2(BPSCat.#60184) 
  • LIGHT,His-Tag(Human)(BPSCat.#71266)
  • LIGHT-CHORecombinantCellLine(BPSCat.#79262) 
  • 96-welltissueculture-treatedwhiteclear-bottomassayplate
  • One-Stepluciferaseassaysystem(BPSbioscience#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
  • Luminometer
Synonym(s):
HVEM,TNFRSF14,CD270,HVEA,TR2,LIGHTR
SuppliedAs:
Eachvialcontains~2x10^6cellsin1mlof10%DMSO.
HostCellline:
JurkatTcell
MycoplasmaTesting:
ThiscelllinehasbeenscreenedusingtheMycoAlert™MycoplasmaDetectionKit(Lonza,#LT07-118)toconfirmtheabsenceofMycoplasmacontamination.MycoAlertAssayControlSet(Lonza,#LT07-518)wasusedasapositivecontrol.
GrowthMedia:

ThawMedium2(BPSCat.#60184):RPMI1640medium(LifeTechnologies#A10491-01)supplementedwith10%FBS(LifeTechnologies#26140-079),1%Penicillin/Streptomycin(Hyclone#SV30010.01)

CompleteGrowthMedium:ThawMedium2(BPSCat.#60184)plus1mg/mlG418(ThermoFisher,Cat.#11811031)and200μg/mlofHygromycinB(Hyclone#SV30070.01)

Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmedium(ThawMedium2withG418andHygromycinB).

Instructionsforuse:
Seedatasheetfordetailedculturingandassayprotocol.
Storage/StABIlity:
Storeinliquidnitrogenimmediatelyuponreceipt.
Application(s):
•ScreenforagonistsorantagonistsofLIGHT-HVEMsignalinginaphysiologicalrelevantcellularcontext

•CharacterizeTcell-mediatedimmuneresponsesofHVEManditsinteractionswithLIGHT

•Screenco-stimulatoryimmunecheckpointmolecules

Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermittheuseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Immunotherapy
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。