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Bpsbioscience/HVEM/NF-κB Reporter Jurkat Recombinant Cell Line/79310/2 vials
RecombinantclonalstableJurkatTcelllineexpressingfireflyluciferasegeneunderthecontrolof4copiesofNF-κBresponseelementswithconstitutiveexpressionofhumanHVEM(HerpesVirusEntryMediator,TumorNecrosisFactorReceptorSuperfamilyMember14,TNFRSF14,CD270,GenBankAccessionNo.NM_003820).FollowingactivationbyhumanHVEMligandLIGHT,NF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasereportergene.
- AssayMedium:ThawMedium2(BPSCat.#60184)
- LIGHT,His-Tag(Human)(BPSCat.#71266)
- LIGHT-CHORecombinantCellLine(BPSCat.#79262)
- 96-welltissueculture-treatedwhiteclear-bottomassayplate
- One-Stepluciferaseassaysystem(BPSbioscience#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
- Luminometer
ThawMedium2(BPSCat.#60184):RPMI1640medium(LifeTechnologies#A10491-01)supplementedwith10%FBS(LifeTechnologies#26140-079),1%Penicillin/Streptomycin(Hyclone#SV30010.01)
CompleteGrowthMedium:ThawMedium2(BPSCat.#60184)plus1mg/mlG418(ThermoFisher,Cat.#11811031)and200μg/mlofHygromycinB(Hyclone#SV30070.01)
Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmedium(ThawMedium2withG418andHygromycinB).
•CharacterizeTcell-mediatedimmuneresponsesofHVEManditsinteractionswithLIGHT
•Screenco-stimulatoryimmunecheckpointmolecules
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LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermittheuseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.