ThawMedium1(BPSBioscience,#60187):MEMmedium(Hyclone,#SH30024.01)+10%FBS(LifeTechnologies,#26140-079)+1%non-essentialaminoacids(Hyclone,#SH30238.01)+1mMNapyruvate(Hyclone,#SH30239.01)+1%Penicillin/Streptomycin(Hyclone,SV30010.01)
CompleteGrowthMedium:ThawMedium1(BPSBioscience,#60187) plus100µg/mlofHygromycinB(LifeTechnologies,#10687-010). Cellsshouldbegrownat37°Cwith5%CO2usingcompletegrowthmediumtoensurerecombinantexpression.CTLA4-HEK293cellsshoulddisplayatypicalcelldivisiontimeofabout24hours.
Immediatelyuponreceipt,storeinliquidnitrogen.
•BindingstudiesusingHVEMligands
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LicenseDisclosure: Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermittheuseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails. PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
