ThawMedium3(BPSCat.#60186):Ham’sF-12medium(Hyclone#SH30526.01)supplementedwith10%FBS(Lifetechnologies#26140-079),1%Penicillin/Streptomycin(HycloneSV30010.01).
CompleteGrowthMedium:ThawMedium3(BPSCat.#60186) plus1mg/mlG418(ThermoFisher,Cat.#11811031)
Cellsshouldbegrownat37°Cwith5%CO2usingThawMedium3(BPSCat.#60186)plus1mg/mlofG418(ThermoFisher,Cat.#11811031)
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