ThawMedium1(BPSBioscience#60187):MEMmedium(Hyclone#SH30024.01)supplementedwith10%FBS(Invitrogen#26140-079),1%non-essentialaminoacids(Hyclone#SH30238.01),1mMNapyruvate(Hyclone#SH30239.01),1%Penicillin/Streptomycin(HycloneSV30010.01)

CompleteGrowthMedium:ThawMedium1(BPSBioscience#60187)plus100µg/mlHygromycinB(ThermoFisher,#10687010).

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